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Human VCAM-1/CD106 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5649A

R&D Systems, part of Bio-Techne
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FAB5649A

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # IE10

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human VCAM-1/CD106 APC-conjugated Antibody

Detection of VCAM-1/CD106 antibody in HuT 78 Human Cell Line antibody by Flow Cytometry.

Detection of VCAM‑1/CD106 in HuT 78 Human Cell Line by Flow Cytometry.

HuT 78 human cutaneous T cell lymphoma cell line was stained with Mouse Anti-Human VCAM-1/CD106 APC-conjugated Monoclonal Antibody (Catalog # FAB5649A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human VCAM-1/CD106 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HuT 78 human cutaneous T cell lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VCAM-1/CD106

Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha4 beta7 to promote leukocyte adhesion at sites of inflamed vasculature.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for Human VCAM-1/CD106 APC-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VCAM-1/CD106 APC-conjugated Antibody

For research use only

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