Human VEGFR2/KDR/Flk-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3571
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Porcine, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Drug Delivery, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 89115
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR2
Ala20-Glu764
Accession # P35968
Ala20-Glu764
Accession # P35968
Specificity
Detects human VEGFR2 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) VEGFR1, rhVEGFR3, or recombinant mouse VEGFR2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human VEGFR2/KDR/Flk-1 Antibody
VEGFR2/KDR/Flk‑1 in Human Placenta.
VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 25 µg/mL Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
VEGFR2/KDR/Flk‑1 in Human Kidney.
VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human kidney array using Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
VEGFR2/KDR/Flk‑1 in HUVECs and Human HeLa Cell Line.
VEGFR2/KDR/Flk-1 was detected in immersion fixed human umbilical vein endothelial cells (HUVECs; left panel) but is not detected in Hela human cervical epithelial carcinoma cell line (right panel) using Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of HUVECs. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human VEGFR2/KDR/Flk-1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed human umbilical vein endothelial cells
Sample: Immersion fixed human umbilical vein endothelial cells
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta and human kidney array
Sample: Immersion fixed paraffin-embedded sections of human placenta and human kidney array
Western Blot
1 µg/mL
Sample: Recombinant Human VEGFR2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD)
under non-reducing conditions only. It is recommended to use Goat Anti-Human VEGFR2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357)
Sample: Recombinant Human VEGFR2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD)
under non-reducing conditions only. It is recommended to use Goat Anti-Human VEGFR2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB3571 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGFR2/KDR/Flk-1
VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1), and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.
VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.
References
- Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Alternate Names
CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2
Gene Symbol
KDR
UniProt
Additional VEGFR2/KDR/Flk-1 Products
Product Documents for Human VEGFR2/KDR/Flk-1 Antibody
Product Specific Notices for Human VEGFR2/KDR/Flk-1 Antibody
For research use only
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