Human VIP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6079
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 576721
Product Specifications
Immunogen
KLH-coupled human VIP synthetic peptides HSDAVFTDNYTR and KYLNSILN
Specificity
Detects recombinant human VIP in direct ELISAs. It does not detect the C-terminal region (aa 145-152).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human VIP Antibody
VIP in Human Stomach.
VIP was detected in immersion fixed paraffin-embedded sections of human stomach array using Mouse Anti-Human VIP Monoclonal Antibody (Catalog # MAB6079) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human VIP Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion-fixed paraffin-embedded sections of human stomach and human stomach cancer tissue
Sample: Immersion-fixed paraffin-embedded sections of human stomach and human stomach cancer tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB6079 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VIP
Vasoactive intestinal peptide (VIP) is a 28 amino acid (aa) peptide that exerts wide-ranging effects in embryonic and adult tissues through interactions with the 7‑transmembrane spanning receptors VIP R1 and VIP R2. The VIP proprotein is proteolytically processed to release bioactive VIP as well as the bioactive peptides PHV and PHM. VIP regulates appetite and feeding behavior and stimulates insulin and glucagon secretion in pancreatic islet cells. It also regulates astrocyte function, multiple aspects of cardiac function, and TLR function in innate immune responses. Mature VIP shares 100% aa sequence identity with mouse and rat VIP.
Long Name
Vasoactive Intestinal Peptide
Alternate Names
MGC13587
Entrez Gene IDs
7432 (Human)
Gene Symbol
VIP
Additional VIP Products
Product Documents for Human VIP Antibody
Product Specific Notices for Human VIP Antibody
For research use only
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