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Human VSIG3 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB92293G

R&D Systems, part of Bio-Techne
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FAB92293G-100UG

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 973422

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VSIG3
Met1-Gly245
Accession # Q5DX21

Specificity

Detects human VSIG3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human VSIG3 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HEK293 Human Cell Line transfected with human VSIG3 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitution default not known

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VSIG3

VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.

Long Name

V-Set and Immunoglobulin Domain Containing 3

Alternate Names

BTIGSF, CXADRL1, IGSF11

Entrez Gene IDs

152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)

Gene Symbol

IGSF11

UniProt

Additional VSIG3 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VSIG3 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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