Human Wnt-6 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4109
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Wnt-6
Met35-Glu75 and Gln238-Arg299
Accession # Q9Y6F9
Met35-Glu75 and Gln238-Arg299
Accession # Q9Y6F9
Specificity
Detects human Wnt-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Wnt-4, rmWnt-8a, and rmWnt-9b is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Wnt-6 Antibody
Wnt-6 in Human Colon Cancer Tissue.
Wnt-6 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Sheep Anti-Human Wnt-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4190) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Wnt-6 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human normal ileum and colon cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human normal ileum and colon cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
0.1 µg/mL
Sample: Recombinant Human Wnt-6
Sample: Recombinant Human Wnt-6
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-6
Human Wnt-6 is a 46 kDa, secreted, glycoprotein of the Wnt family. Human Wnt-6 is synthesized as a 365 amino acid (aa) precursor that contains a 341 aa mature region. The mature molecule contains 24 cysteines and one potential N-linked glycosylation site. Wnt-6 is produced by mammary fibroblasts and ductal epithelium, intestinal crypt cells, and limb bud ectoderm. Over the regions used as immunogen, the aa sequences of human, mouse, and canine Wnt-6 are identical.
Long Name
Wingless-type MMTV Integration Site Family, Member 6
Alternate Names
Wnt6
Gene Symbol
WNT6
UniProt
Additional Wnt-6 Products
Product Documents for Human Wnt-6 Antibody
Product Specific Notices for Human Wnt-6 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...