Human Wnt-9a Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB31571
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 326707
Product Specifications
Immunogen
E. coli-derived recombinant human Wnt-9a
Pro48-Glu92 & Arg231-Phe304
Accession # O14904.2
Pro48-Glu92 & Arg231-Phe304
Accession # O14904.2
Specificity
Detects recombinant human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -2b, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Wnt-9a Antibody
Wnt-9a in Human Breast Cancer Tissue.
Wnt-9a was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Mouse Anti-Human Wnt-9a Monoclonal Antibody (Catalog # MAB31571) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Wnt-9a Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
1 µg/mL
Sample: Recombinant Human Wnt-9a
under non-reducing conditions only
Sample: Recombinant Human Wnt-9a
under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4 using MAB31571 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-9a
Long Name
Wingless-type MMTV Integration Site Family, Member 9A
Alternate Names
Wnt-14, Wnt9a
Gene Symbol
WNT9A
UniProt
Additional Wnt-9a Products
Product Documents for Human Wnt-9a Antibody
Product Specific Notices for Human Wnt-9a Antibody
For research use only
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