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Human XCR1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF857

R&D Systems, part of Bio-Techne
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BAF857

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human XCR1
Met1-Thr31, Ser89-Lys103, His168-Val190, Phe251-Tyr267
Accession # P46094

Specificity

Detects human XCR1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human XCR1 Biotinylated Antibody

Detection of XCR1 antibody in Human LPS-treated PBMCs antibody by Flow Cytometry.

Detection of XCR1 in Human LPS-treated PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells were treated for 16 hours with 1 µg/mL LPS then stained with Goat Anti-Human XCR1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF857, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Applications for Human XCR1 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells treated with LPS

Western Blot

0.1 µg/mL
Sample: Recombinant Human XCR1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XCR1

XCR1, also known as GPR5 and lymphotactin/SCM-1 (single cysteine motif 1) receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, human herpesvirus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells , NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.

Long Name

Chemokine [C Motif] Receptor 1

Alternate Names

CCXCR1, GPR5

Entrez Gene IDs

2829 (Human); 23832 (Mouse); 301086 (Rat)

Gene Symbol

XCR1

UniProt

Additional XCR1 Products

Product Documents for Human XCR1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human XCR1 Biotinylated Antibody

For research use only

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