Human YY1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3784
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human YY1
His75-Gln275
Accession # P25490
His75-Gln275
Accession # P25490
Specificity
Detects human YY1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human YY1 Antibody
Detection of Human YY1 by Western Blot.
Western blot shows nuclear extracts of Daudi human Burkitt's lymphoma cell line, U937 human histiocytic lymphoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human YY1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3784) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for YY1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
YY1 in Human Breast.
YY1 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human YY1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3784) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human YY1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast
Sample: Immersion fixed paraffin-embedded sections of human breast
Western Blot
0.5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, U937 human histiocytic lymphoma cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: Daudi human Burkitt's lymphoma cell line, U937 human histiocytic lymphoma cell line, and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: YY1
YY1 (Yin Yang 1) is a multifunctional transcription factor belonging to the GLI-Kruppel class of zing finger proteins. YY1 is essential for embryogenesis, apoptosis, differentiation, cell growth and proliferation. YY1 binds to the DNA consensus site CGCCATNTT. YY1 can function as an initiator of transcription as well as activating or repressing transcription through protein complexes (e.g. histone deacetylases and histone acetyltransferases).
Long Name
Yin-Yang-1
Alternate Names
DELTA, NF-E1, UCRBP
Gene Symbol
YY1
UniProt
Additional YY1 Products
Product Documents for Human YY1 Antibody
Product Specific Notices for Human YY1 Antibody
For research use only
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