A549 human lung carcinoma cell line was stained with Mouse Anti-Human ZEB2/SIP1 Monoclonal Antibody (Catalog # MAB73782, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

ZEB2/SIP1 in Human Liver.

ZEB2/SIP1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human ZEB2/SIP1 Monoclonal Antibody (Catalog # MAB73782) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ZEB2/SIP1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: A549 human lung carcinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZEB2/SIP1

SIP1 (Smad-interacting protein 1), also called ZEB2 (Zinc finger E-box-binding homeobox 2), is a nuclear transcription factor. SIP1 contains seven C2H2-type zinc finger domains within amino acids (aa) 211-334 and 999-1076, a Smad-MHZ binding domain (aa 437-487), an atypical homeobox domain (aa 644-703), and phosphorylation, sumoylation and acetylation sites. The 1214 aa SIP1 gives a calculated molecular weight of 136 kDa, but may actually appear closer to 200 kDa due to modifications. A 1190 aa isoform lacks aa 111-134. Within aa 363-537, human SIP1 shares 98% and 97% aa sequence identity with mouse and rat SIP1, respectively. SIP1 is highly expressed in postmitotic neocortical cells and influences cell fate decisions in embryonic brain development. Point mutations causing underexpression of SIP1 are associated with Mowat-Wilson syndrome (MWIS), also known as Hirschprung disease mental retardation syndrome.

Alternate Names

HSPC082, SIP1, SMADIP1, ZFHX1B

Entrez Gene IDs

9839 (Human); 24136 (Mouse); 311071 (Rat)

Gene Symbol

ZEB2

UniProt

O60315 (Human)

Additional ZEB2/SIP1 Products

Product Documents for Human ZEB2/SIP1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ZEB2/SIP1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ZEB2/SIP1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB73782

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ZEB2/SIP1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ZEB2/SIP1 Antibody

Detection of ZEB2/SIP1 in A549 Human Cell Line by Flow Cytometry.