Human ZNF24 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3637

R&D Systems, part of Bio-Techne
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AF3637
AF3637-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP), Functional Assay, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all ZNF24 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZNF24
Ser2-Val368
Accession # NP_008896

Specificity

Detects human ZNF24 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ZNF24 Antibody

ZNF24 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

ZNF24 in HepG2 Human Cell Line.

ZNF24 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human ZNF24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3637) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human ZNF24 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human ZNF24

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZNF24

Human ZNF24, also known as ZNF191, is a 42 kDa transcription regulator that belongs to the Kruppel-like zinc finger gene family. It contains an N-terminal SCAN box domain and four C-terminal tandem zinc finger motifs. ZNF24 is ubiquitously expressed. A second splice isoform that contains a 4 residue substitution for aa 191‑368 of full-length ZNF24, has been reported. Human and mouse ZNF24 share 95% amino acid sequence homology.

Long Name

Zinc Finger Protein 24

Alternate Names

KOX17, RSG-A, Zfp191, ZSCAN3

Entrez Gene IDs

7572 (Human); 331360 (Mouse)

Gene Symbol

ZNF24

UniProt

NP_008896

Additional ZNF24 Products

Product Documents for Human ZNF24 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ZNF24 Antibody

For research use only

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