Human ZNF281 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6350
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 801706
Product Summary for Human ZNF281 Antibody
Immunogen
E. coli-derived recombinant human ZNF281
Asn681-Arg895
Accession # Q9Y2X9
Asn681-Arg895
Accession # Q9Y2X9
Specificity
Detects human ZNF281 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human ZNF281 Antibody
Detection of Human ZNF281 by Western Blot.
Western blot shows lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ZNF281 Monoclonal Antibody (Catalog # MAB6350) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ZNF281 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ZNF281 in BG01V Human Embryonic Stem Cells.
ZNF281 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human ZNF281 Monoclonal Antibody (Catalog # MAB6350) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Human ZNF281 by Simple WesternTM.
Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.5 mg/mL. A specific band was detected for ZNF281 at approximately 107 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human ZNF281 Monoclonal Antibody (Catalog # MAB6350). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Applications for Human ZNF281 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Simple Western
50 µg/mL
Sample: BG01V human embryonic stem cells
Sample: BG01V human embryonic stem cells
Western Blot
1 µg/mL
Sample: BG01V human embryonic stem cells
Sample: BG01V human embryonic stem cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZNF281
Long Name
Zinc Finger Protein 281
Alternate Names
GZP1, ZBP-99, ZNP-99
Gene Symbol
ZNF281
UniProt
Additional ZNF281 Products
Product Documents for Human ZNF281 Antibody
Product Specific Notices for Human ZNF281 Antibody
For research use only
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