Human ZnT-8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7318
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ZnT-8
Lys268-Pro359
Accession # Q8IWU4
Lys268-Pro359
Accession # Q8IWU4
Specificity
Detects human Znt-8 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ZnT-8 Antibody
ZnT-8 in Human Pancreas.
ZnT-8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human ZnT-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7318) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human ZnT-8 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013).
Sample: Immersion fixed paraffin-embedded sections of human pancreas subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013).
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZnT-8
ZnT-8 (Zinc Transporter 8; also SLC30A8) is a 35-40 kDa member of the SLC (solute carrier)-30A subfamily, CDF family of proteins. It is expressed by pancreatic beta‑cells and alpha-cells, B cells, and adipocytes, and is known to play a role in Zn transport. In particular, ZnT-8 appears to transport zinc from the cytosol into secretory vesicles which, in the case of beta-cells, provides a necessary component for proper insulin processing and granule storage. Further, it appears to facilitate glucose‑mediated insulin release. Human ZnT-8 is a multipass transmembrane (TM) protein that is 369 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-79) followed by six TM segments (aa 80-266) and a 103 aa C-terminal cytoplasmic tail. There is a key His-rich motif in the second cytoplasmic loop (aa 197‑205). ZnT-8 forms homodimers, and possibly oligomers. There is one alternative start site at Met50. Over aa 268-359, human ZnT-8 shares 79% aa sequence identity with mouse ZnT-8.
Long Name
Zinc Transporter 8
Alternate Names
SLC30A8, ZnT8
Gene Symbol
SLC30A8
UniProt
Additional ZnT-8 Products
Product Documents for Human ZnT-8 Antibody
Product Specific Notices for Human ZnT-8 Antibody
For research use only
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