Humanin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB300-245
![Western Blot: Humanin AntibodyBSA Free [NB300-245]](https://resources.bio-techne.com/images/products/Humanin-Antibody-Western-Blot-NB300-245-img0002.jpg "Western Blot: Humanin AntibodyBSA Free [NB300-245]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Residues 9-24 (LLLLTSEIDLPVKRRA) of the human Humanin protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Humanin Antibody - BSA Free
Western Blot: Humanin AntibodyBSA Free [NB300-245]
Western Blot: Humanin Antibody [NB300-245] - 100ng of synthetic Humanin peptide and from 50 ug of total lysates of 293 T cells transfected with plasmids encoding GFPc1 or GFP-Humanin Conditions for the Western Blot: Lysis buffer: (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 20 mM EDTA, 50 mM NaF, 0.5% NP-40, 0.1 mM Na3VO4, 20 ug ml-1 leupeptin, 20 ug ml-1 aprotinin, 1 mM dithiothreitol and 1 mM phenylmethyl sulphonyl fluoride (PMSF)). Blocking buffer: (TRiS/Sodium chloride/buffer pH7.6) with 0.1% Tween-20, 5% skim Milk and 2% BSA). Humanin primary antibody(dilution 1/1000) and GFP antibody (dilution 1/2000) were incubated ON at 4 degrees C. Secondary rabbit HRP antibody was incubated during 1 hour at RT following by ECL detection.Applications for Humanin Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
This antibody may be used for Western blot. No other assays have been tested. Suggested working dilutions: The investigator should determine the optimal working dilution for a specific application.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Humanin
Alternate Names
HN, HUNIN
Gene Symbol
MTRNR2L1
Additional Humanin Products
Product Documents for Humanin Antibody - BSA Free
Product Specific Notices for Humanin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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