Huntingtin Antibody (7B9C8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15642

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15642-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7B9C8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Huntingtin Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Huntingtin (P42858). MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPKKELSATKKDRV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Huntingtin Antibody (7B9C8)

Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642]

Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642]

Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry: Huntingtin Antibody (7B9C8) [NBP3-15642]

Immunohistochemistry: Huntingtin Antibody (7B9C8) [NBP3-15642]

Immunohistochemistry: Huntingtin Antibody (7B9C8) [NBP3-15642] - Immunofluorescence analysis of mouse brain using Huntingtin Rabbit mAb (NBP3-15642) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642]

Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642]

Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
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Applications for Huntingtin Antibody (7B9C8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Huntingtin

Huntingtin protein (Htt) is a 348 kDa protein product of Huntington's disease (HD, IT15) gene. Huntington's disease is a neurodegenerative disorder caused by a mutation on the HD gene, producing a polyglutamin (polyQ) expansion on the N-terminus of Htt. The mutant Htt leads to cytotoxicity in the striatal neuron (1). This cytotoxicity is modulated by proteolytic cleavage with caspases and calpains, producing N-terminal polyQ fragments. A phosphorylation of Htt may also regulate the cleavage and cytotoxicity of mutant Htt (2). The wild-type Htt up-regulates transcription of brain-derived neurotrophic factor (BDNF), a pro-survival factor for striatal neurons. This suggests restoring wild-type Htt level and increasing BDNF expression level can be therapeutic treatment for Huntington's disease (3). Huntingtin is also cleaved by apopain, a cysteine protease product of death-gene for human, suggesting HD might be a disorder of inappropriate apoptosis (4).

Long Name

Huntingtin

Alternate Names

HD, HTT, IT15

Gene Symbol

HTT

Additional Huntingtin Products

Product Documents for Huntingtin Antibody (7B9C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Huntingtin Antibody (7B9C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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