Huntingtin Antibody (HDB4E10)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1197
Key Product Details
Species Reactivity
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # HDB4E10
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant protein corresponding to amino acids 1844 - 2131 of huntingtin
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Cytoplasmic
Specificity
This reacts with an epitope corresponding to the HDB region (amino acids 1844 - 2131) of the huntingtin protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Huntingtin Antibody (HDB4E10)
![Western Blot: Huntingtin Antibody (HDB4E10)BSA Free [NB600-1197]](https://resources.bio-techne.com/images/products/Huntingtin-Antibody-HDB4E10-Western-Blot-NB600-1197-img0001.jpg "Western Blot: Huntingtin Antibody (HDB4E10)BSA Free [NB600-1197]")
Western Blot: Huntingtin Antibody (HDB4E10)BSA Free [NB600-1197]
Western Blot: Huntingtin Antibody (HDB4E10) [NB600-1197] - Western Blot analysis of normal human cerebral cortex total protein extract run on a 3-12.5% gradient SDS-PAGE gel and probed with Mouse anti HuntingtinApplications for Huntingtin Antibody (HDB4E10)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
Immunohistology on frozen (paraffin not tested). Western blotting detects a 350kD band on western blots but also detects smaller degradation products of huntingtin. This clone recognizes both denatured and native huntingtin in human brain.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Huntingtin
Long Name
Huntingtin
Alternate Names
HD, HTT, IT15
Gene Symbol
HTT
UniProt
Additional Huntingtin Products
Product Documents for Huntingtin Antibody (HDB4E10)
Product Specific Notices for Huntingtin Antibody (HDB4E10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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