Huntingtin Antibody (JB89-34)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75531

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-75531
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JB89-34

Concentration

1 mg/ml

View all Huntingtin Antibodies »

Product Specifications

Immunogen

Synthetic peptide within N-terminal Human Huntingtin . (SwissProt: P42858 Human; SwissProt: P42859 Mouse; SwissProt: P51111 Rat)

Localization

Cytoplasm. Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

347 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Huntingtin Antibody (JB89-34)

Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531]

Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531]

Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate
Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531]

Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531]

Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.
Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531]

Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531]

Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Applications for Huntingtin Antibody (JB89-34)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Huntingtin

Huntingtin protein (Htt) is a 348 kDa protein product of Huntington's disease (HD, IT15) gene. Huntington's disease is a neurodegenerative disorder caused by a mutation on the HD gene, producing a polyglutamin (polyQ) expansion on the N-terminus of Htt. The mutant Htt leads to cytotoxicity in the striatal neuron (1). This cytotoxicity is modulated by proteolytic cleavage with caspases and calpains, producing N-terminal polyQ fragments. A phosphorylation of Htt may also regulate the cleavage and cytotoxicity of mutant Htt (2). The wild-type Htt up-regulates transcription of brain-derived neurotrophic factor (BDNF), a pro-survival factor for striatal neurons. This suggests restoring wild-type Htt level and increasing BDNF expression level can be therapeutic treatment for Huntington's disease (3). Huntingtin is also cleaved by apopain, a cysteine protease product of death-gene for human, suggesting HD might be a disorder of inappropriate apoptosis (4).

Long Name

Huntingtin

Alternate Names

HD, HTT, IT15

Gene Symbol

HTT

Additional Huntingtin Products

Product Documents for Huntingtin Antibody (JB89-34)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Huntingtin Antibody (JB89-34)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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