Huntingtin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44266
![Western Blot: Huntingtin Antibody [NBP1-44266]](https://resources.bio-techne.com/images/products/135219_1.jpg "Western Blot: Huntingtin Antibody [NBP1-44266]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Huntingtin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human Huntingtin disease protein. (Uniprot: P42858)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, and Rat based on 100% sequence homology. Cross-reactivity with HTT from other sources has not been determined.
Localization
Brain
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Huntingtin Antibody
Western Blot: Huntingtin Antibody [NBP1-44266]
Western Blot: Huntingtin Antibody [NBP1-44266] - Western blot analysis on mouse brain extract using 0.1ug/mL of NBP1-44266 (lane 1) and 0.1ug/mL of NBP1-44266 combined with 1ug/mL of blocking peptide (lane 2).Applications for Huntingtin Antibody
Application
Recommended Usage
ELISA
1:20000-1:60000
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry
1:100-1:500
Western Blot
0.1ug/ml
Application Notes
This product is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: Huntingtin
Long Name
Huntingtin
Alternate Names
HD, HTT, IT15
Entrez Gene IDs
3064 (Human)
Gene Symbol
HTT
UniProt
Additional Huntingtin Products
Product Documents for Huntingtin Antibody
Product Specific Notices for Huntingtin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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