HUS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89445

Novus Biologicals, part of Bio-Techne
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NBP1-89445
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HUS1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRASKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HUS1 Antibody

Western Blot: HUS1 Antibody [NBP1-89445]

Western Blot: HUS1 Antibody [NBP1-89445]

Western Blot: HUS1 Antibody [NBP1-89445] - Analysis in control (vector only transfected HEK293T lysate) and HUS1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: HUS1 Antibody [NBP1-89445]

Immunohistochemistry-Paraffin: HUS1 Antibody [NBP1-89445]

Immunohistochemistry-Paraffin: HUS1 Antibody [NBP1-89445] - Staining of human testis shows nuclear positivity in cells of seminiferus ducts.
Western Blot: HUS1 Antibody [NBP1-89445]

Western Blot: HUS1 Antibody [NBP1-89445]

Western Blot: HUS1 Antibody [NBP1-89445] - Image was provided by Dr. A-Lien Lu-Chang at Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine.

Applications for HUS1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:32991318).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HUS1

The protein encoded by the HUS1 gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event.

Alternate Names

checkpoint protein HUS1, hHUS1, HUS1 (S. pombe) checkpoint homolog, HUS1 checkpoint homolog (S. pombe), hus1+-like protein

Gene Symbol

HUS1

Additional HUS1 Products

Product Documents for HUS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HUS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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