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HUS1B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77749

Novus Biologicals, part of Bio-Techne
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NBP1-77749

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for HUS1B Antibody

Immunogen

This affinity purified HUS1B Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human HUS1B protein. (Uniprot: Q8NHY5)

Reactivity Notes

A BLAST analysis indicates 100% homology of the immunizing sequence with HUS1B protein from human. Partial reactivity with mouse HUS1B is not likely based on low (~64%, 9/14) sequence homology. Reactivity against homologues from other sources is not known.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This affinity-purified antibody is directed against human Hus1B protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for HUS1B Antibody

Western Blot: HUS1B Antibody [NBP1-77749]

Western Blot: HUS1B Antibody [NBP1-77749]

Western Blot: HUS1B Antibody [NBP1-77749] - Shows detection of a 36kDa band corresponding to Hus1B in a HeLa cell lysate (arrowhead). The staining pattern in 293 cells is less clear, showing a predominant band at 39 kDa. Personal Communication, A-Lien Lu-Chang, U. Maryland, Baltimore, MD

Applications for HUS1B Antibody

Application
Recommended Usage

ELISA

1:20000-1:100000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to Hus1B protein by western blotting in the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: HUS1B

HUS1Bis most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. HUS1B can interact with the check point protein RAD1 but not with RAD9. Overexpression of HUS1B has been shown to induce cell death, which suggests a related but distinct role of this protein, as compared to the HUS1.The protein encoded by this gene is most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. This protein can interact with the check point protein RAD1 but not with RAD9. Overexpression of this protein has been shown to induce cell death, which suggests a related but distinct role of this protein, as compared to the HUS1.

Alternate Names

checkpoint protein HUS1B, hHUS1B, HUS1 (S. pombe) checkpoint homolog b, HUS1 checkpoint homolog b (S. pombe), MGC126746, MGC126748, RP11-532F6.1

Gene Symbol

HUS1B

UniProt

Additional HUS1B Products

Product Documents for HUS1B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HUS1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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