HUWE1 Antibody (BLR129H)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14712

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14712
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR129H

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HUWE1 Antibodies »

Product Specifications

Immunogen

between 2250 and 2300

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for HUWE1 Antibody (BLR129H)

Western Blot: HUWE1 Antibody (BLR129H) [NBP3-14712]

Western Blot: HUWE1 Antibody (BLR129H) [NBP3-14712]

Western Blot: HUWE1 Antibody (BLR129H) [NBP3-14712] - Rabbit antiLasu1/Ureb1 recombinant monoclonal antibody [BLR129H] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds.Lower Panel: Rabbit anti-COPB2 antibody.
Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody (BLR129H) [NBP3-14712]

Immunocytochemistry/ Immunofluorescence: HUWE1 Antibody (BLR129H) [NBP3-14712]

Immunocytochemistry/Immunofluorescence: HUWE1 Antibody (BLR129H) [NBP3-14712] - SiHa cells by immunocytochemistry. Antibody: Rabbit anti-Lasu1/Ureb1recombinant monoclonal antibody [BLR129H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB
Immunohistochemistry-Paraffin: HUWE1 Antibody (BLR129H) [NBP3-14712]

Immunohistochemistry-Paraffin: HUWE1 Antibody (BLR129H) [NBP3-14712]

Immunohistochemistry-Paraffin: HUWE1 Antibody (BLR129H) [NBP3-14712] - Head & neck SCC. Rabbit antiLasu1/Ureb1 recombinant monoclonal antibody [BLR129H]. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.
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Applications for HUWE1 Antibody (BLR129H)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

20 ul/1 mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: HUWE1

ARFBP1 is a novel E3 ubiquitin ligase. It interacts with p19ARF and its ligase activity is inhibited by this interaction.

Alternate Names

arf-bp1, EC 6.3.2, EC 6.3.2.6, HECT, UBA and WWE domain containing 1, HECTH9, HSPC272, KIAA0312MULE, Mcl-1 ubiquitin ligase E3, Mule, UREB1UBA and WWE domain-containing protein 1, URE-binding protein 1

Gene Symbol

HUWE1

Additional HUWE1 Products

Product Documents for HUWE1 Antibody (BLR129H)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HUWE1 Antibody (BLR129H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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