Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all HUWE1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ARF-BP1. The immunogen is located within amino acids 4250 - 4300 of ARF-BP1. Amino Acid Squence: IDIDDLKSNTEYHKYQSNS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

481.89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HUWE1 Antibody - BSA Free

Western Blot: HUWE1 AntibodyBSA Free [NBP1-76795]

Western Blot: HUWE1 AntibodyBSA Free [NBP1-76795]

Western Blot: HUWE1 Antibody [NBP1-76795] - Daudi cell lysate with HUWE1 antibody at 1 ug/ml.

Immunocytochemistry/Immunofluorescence: HUWE1 Antibody [NBP1-76795] -

Immunocytochemistry/Immunofluorescence: HUWE1 Antibody [NBP1-76795] - Daudi cells with HUWE1 antibody at 5 ug/ml.

Immunocytochemistry/Immunofluorescence: HUWE1 Antibody [NBP1-76795] -

Immunocytochemistry/Immunofluorescence: HUWE1 Antibody [NBP1-76795] - Daudi cells at 20 ug/ml
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Applications for HUWE1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5-20 ug/mL

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HUWE1

The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.

Alternate Names

arf-bp1, EC 6.3.2, EC 6.3.2.6, HECT, UBA and WWE domain containing 1, HECTH9, HSPC272, KIAA0312MULE, Mcl-1 ubiquitin ligase E3, Mule, UREB1UBA and WWE domain-containing protein 1, URE-binding protein 1

Entrez Gene IDs

10075 (Human)

Gene Symbol

HUWE1

UniProt

Q7Z6Z7 (Human)

Additional HUWE1 Products

Product Documents for HUWE1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HUWE1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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