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HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09029

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-09029-0.025mg
NBP3-09029-0.2mg

Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, Flow Cytometry, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # HMHV-1B18

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

This antibody was raised by immunising Armenian hamsters with mouse HVEM:Fc fusion protein.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free

Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18)ChimericAzide and BSA Free [NBP3-09029]

Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18)ChimericAzide and BSA Free [NBP3-09029]

Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (NBP3-09029) at 0.01 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free [NBP3-09029]

Immunocytochemistry/ Immunofluorescence: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free [NBP3-09029]

Immunocytochemistry/Immunofluorescence: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of HMHV-1B18 (NBP3-09029) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09029, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional Assay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

This antibody has been used in FACS to demonstrate that lymphatic endothelial cells mediate deletion only via programmed cell death-1 (PD-1) ligand 1 (Tewalt et al 2012) and in Western Blot to study the role of LIGHT in the pathogenesis of hepatitis (Anand et al 2006). This antibody has been also been used in vivo experiments to study the mechanisms by which TNFSF14 functions to promote airway remodelling in asthma (Sibilano et al 2016), to confirm that costimulatory role through HVEM is not necessary for LIGHT-mediated liver inflammation (Anand et at 2006), and to investigate the role that herpesvirus entry mediator plays in the development of experimental conjunctivitis (Ishida et al, 2012). Treatment with this antibody has been observed to diminish plasma levels of antigen-specific IgG1 and IgE antibodies in mouse asthma models (Sibilano et al 2016), to interfere with the LIGHT-HVEM interaction but not interaction between B and T lymphocyte attenuator (BTLA) and HVEM in mouse hepatitis models (Anand et at 2006), and NOT to affect the development of experimental conjunctivitis in either the induction or the effector phase (Ishida et al, 2012).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HVEM/TNFRSF14

TNFRSF14 is encoded by this gene is a member of the TNF-receptor superfamily. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of this receptor was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response.

Long Name

Herpesvirus Entry Mediator

Alternate Names

ATAR, CD270, LIGHTR, TNFRSF14

Gene Symbol

TNFRSF14

Additional HVEM/TNFRSF14 Products

Product Documents for HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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