Hydrogen Potassium ATPase Beta Antibody (2G11)

Novus Biologicals, part of Bio-Techne | Catalog # NB300-583

Novus Biologicals, part of Bio-Techne
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NB300-583
NB300-583-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Ferret, Rabbit

Cited:

Mouse, Rat

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Dot Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Cytometric Bead Assay Standard, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2G11

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Hydrogen Potassium ATPase Beta Antibodies »

Product Specifications

Immunogen

Purified 34 kDa core peptide from deglycosylated hog gastric microsomes.

Epitope

This antibody recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Detects the beta-subunit of hydrogen/potassium ATPase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Hydrogen Potassium ATPase Beta Antibody (2G11)

Immunocytochemistry/ Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583]

Immunocytochemistry/ Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583]

Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583]

Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583]

Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human stomach tissues.
Immunocytochemistry/ Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583]

Immunocytochemistry/ Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583]

Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Applications for Hydrogen Potassium ATPase Beta Antibody (2G11)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Dot Blot

1:100 - 1:2000

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:2000

Immunohistochemistry-Paraffin

1:2000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:4000
Application Notes
DB usage was reported in scientific literature (PMID: 26686297).

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites diluted with PBS

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hydrogen Potassium ATPase Beta

The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha-subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.

Long Name

Potassium-transporting ATPase subunit beta

Alternate Names

ATP4B

Entrez Gene IDs

11944 (Mouse); 24216 (Rat)

Gene Symbol

ATP4A

Additional Hydrogen Potassium ATPase Beta Products

Product Documents for Hydrogen Potassium ATPase Beta Antibody (2G11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hydrogen Potassium ATPase Beta Antibody (2G11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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