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HYI Antibody (2F6)

Novus Biologicals, part of Bio-Techne | Catalog # H00081888-M02

Novus Biologicals, part of Bio-Techne
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H00081888-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HYI (AAH06140, 1 a.a. ~ 217 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGLGAVPGRQAAFREGLEQAVRYAKALGCPRIHLMAGRVPQGADRIAVKAEMEAVFLENLRHAAGVLAQEDLVGLLEPINTRITDPQYFLDTPQQAAAILQKVGRPNLQLQMDIFHWQIMDGNLTGNIREFLPIVGHVQVAQVPGRGEPSSPGELNFPYLFQLLENEGYKGFVGCEYQPRGDTVEGLSWLRSYWDRRATQRLASEGPHTTHVPPDSE

Reactivity Notes

Human. Other species not tested.

Specificity

HYI - hydroxypyruvate isomerase homolog (E. coli)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for HYI Antibody (2F6)

Western Blot: HYI Antibody (2F6) [H00081888-M02]

Western Blot: HYI Antibody (2F6) [H00081888-M02]

Western Blot: HYI Antibody (2F6) [H00081888-M02] - HYI monoclonal antibody (M02), clone 2F6 Analysis of HYI expression in HL-60.
Sandwich ELISA: HYI Antibody (2F6) [H00081888-M02] - Detection limit for recombinant GST tagged HYI is approximately 3ng/ml as a capture antibody.

Applications for HYI Antibody (2F6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HYI

HYI (Hydroxypyruvate isomerase homolog) is a putative hydroxypyruvate isomerase. Hydroxypyruvate isomerase catalyzes the conversion of hydroxypyruvate to 2-hydroxy-3-oxopropanoate.

Alternate Names

EC 5.3.1.22, Endothelial cell apoptosis protein E-CE1, HT036, hydroxypyruvate isomerase (putative), hydroxypyruvate isomerase homolog, hydroxypyruvate isomerase homolog (E. coli), MGC20767, putative hydroxypyruvate isomerase

Entrez Gene IDs

81888 (Human)

Gene Symbol

HYI

Additional HYI Products

Product Documents for HYI Antibody (2F6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HYI Antibody (2F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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