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HYI Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81659

Novus Biologicals, part of Bio-Techne
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NBP1-81659
NBP1-81659-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALGCPRIHLMAGRVPQGADRIAVKAEMEAVFLENLRHAAGVLAQEDLVGLLEPINTRITDPQYFLDTPQQAAAILQKVGRPNLQLQMDIFHWQIMDGNLTGNIREFLPIVGHVQVAQVPGRGEPSSPGELNFPYLFQLLEDEGYKGFVGC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HYI Antibody

Western Blot: HYI Antibody [NBP1-81659]

Western Blot: HYI Antibody [NBP1-81659]

Western Blot: HYI Antibody [NBP1-81659] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: HYI Antibody [NBP1-81659]

Immunocytochemistry/ Immunofluorescence: HYI Antibody [NBP1-81659]

Immunocytochemistry/Immunofluorescence: HYI Antibody [NBP1-81659] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunohistochemistry: HYI Antibody [NBP1-81659]

Immunohistochemistry: HYI Antibody [NBP1-81659]

Immunohistochemistry: HYI Antibody [NBP1-81659] - Staining of human ovary shows cytoplasmic positivity in ovarian stroma cells.

Applications for HYI Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HYI

Alternate Names

EC 5.3.1.22, Endothelial cell apoptosis protein E-CE1, HT036, hydroxypyruvate isomerase (putative), hydroxypyruvate isomerase homolog, hydroxypyruvate isomerase homolog (E. coli), MGC20767, putative hydroxypyruvate isomerase

Gene Symbol

HYI

Additional HYI Products

Product Documents for HYI Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HYI Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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