I-Ab Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15581
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-219 of mouse I-Ab (NP_996988.2). SERHFVYQFMGECYFTNGTQRIRYVTRYIYNREEYVRYDSDVGEHRAVTELGRPDAEYWNSQPEILERTRAELDTVCRHNYEGPETHTSLRRLEQPNVVISLSRTEALNHHNTLVCSVTDFYPAKIKVRWFRNGQEETVGVSSTQLIRNGDWTFQVLVMLEMTPRRGEVYTCHVEHPSLKSPITVEWRAQ
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for I-Ab Antibody - Azide and BSA Free
![Immunocytochemistry/ Immunofluorescence: I-Ab Antibody - Azide and BSA Free [NBP3-15581]](https://resources.bio-techne.com/images/products/I-Ab-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15581-img0002.jpg "Immunocytochemistry/ Immunofluorescence: I-Ab Antibody - Azide and BSA Free [NBP3-15581]")
Immunocytochemistry/ Immunofluorescence: I-Ab Antibody - Azide and BSA Free [NBP3-15581]
Immunocytochemistry/Immunofluorescence: I-Ab Antibody [NBP3-15581] - Immunofluorescence analysis of NIH-3T3 cells using I-Ab Rabbit pAb (NBP3-15581) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: I-Ab Antibody [NBP3-15581] -
Western Blot: I-Ab Antibody [NBP3-15581] - Analysis of Mouse large intestine, using H2-Ab1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.
Immunohistochemistry-Paraffin: I-Ab Antibody [NBP3-15581] -
Immunohistochemistry-Paraffin: I-Ab Antibody [NBP3-15581] -Mouse spleen using H2-Ab1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Applications for I-Ab Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:100 - 1:500
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: I-Ab
Alternate Names
MHC class II Ab
Gene Symbol
H2-Ab1
Additional I-Ab Products
Product Documents for I-Ab Antibody - Azide and BSA Free
Product Specific Notices for I-Ab Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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