IARS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84637

Novus Biologicals, part of Bio-Techne
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NBP1-84637
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IARS2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALNGMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIVKLVEQHGSDIWWTLPPEQLLPKEVLSEVGGPDALEYVPGQD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IARS2 Antibody

Western Blot: IARS2 Antibody [NBP1-84637]

Western Blot: IARS2 Antibody [NBP1-84637]

Western Blot: IARS2 Antibody [NBP1-84637] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: IARS2 Antibody [NBP1-84637]

Immunocytochemistry/ Immunofluorescence: IARS2 Antibody [NBP1-84637]

Immunocytochemistry/Immunofluorescence: IARS2 Antibody [NBP1-84637] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: IARS2 Antibody [NBP1-84637]

Immunohistochemistry-Paraffin: IARS2 Antibody [NBP1-84637]

Immunohistochemistry-Paraffin: IARS2 Antibody [NBP1-84637] - Staining of human colon shows strong cytoplasmic positivity with a granular pattern in glandular cells.
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Applications for IARS2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IARS2

The IARS2 gene encodes a 1,012 amino acid long, 113 kDA isoleucine-tRNA ligase, mitochondrial that participates in gene expression, mitochondrial tRNA aminoacylation and biosynthesis, as well as valine, leucine, and isoleucine biosynthesis. IARS2 has been linked to diabetes mellitus, cardiomyopathy, and pneumonia.

Alternate Names

EC 6.1.1, EC 6.1.1.5, FLJ10326, IleRS, isoleucine tRNA ligase 2, mitochondrial, Isoleucine--tRNA ligase, isoleucine-tRNA synthetase 2, mitochondrial, isoleucyl-tRNA synthetase 2, mitochondrial, isoleucyl-tRNA synthetase, mitochondrial, mitochondrial isoleucine tRNA synthetase

Gene Symbol

IARS2

Additional IARS2 Products

Product Documents for IARS2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IARS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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