ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58732

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1H4

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all ICAM-1/CD54 Antibodies »

Product Specifications

Immunogen

Raji cells and spleen cells fused with NS1 cells.

Specificity

This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free

Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]

Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]

Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Surface staining of human peripheral blood with anti-CD54 (1H4) FITC. Image using the FITC format of this antibody.
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]

Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]

Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Expression of CD54 in normal human peripheral blood, as detected with anti-CD54 (1H4) biotin.
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]

Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free [NB110-58732]

Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Fig. 1. Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells were used for analysis. Image using the Biotin form of this antibody.
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Applications for ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

5-10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry-Paraffin sections recommended dilution: 10 ug/ml Application Note: Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is required.

Western blotting: Recommended dilution: 1-2 ug/ml; non-reducing conditions.

This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

(PBS), approx. pH 7.4; 0.2 um filter sterilized.

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ICAM-1/CD54

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ICAM-1/CD54 Antibody (1H4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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