ICAM-1/CD54 Antibody (J0-F7)

Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896]

Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896]

Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R))488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Flow Cytometry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896]

Flow Cytometry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Flow cytometric analysis of ICAM-1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-6, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a FITC-conjugated Goat anti-Mouse IgG Secondary antibody at 1/200 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).