ICAM-2/CD102 Antibody (JE63-28)

Western Blot: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456]

Western Blot: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Western blot analysis of ICAM-2/CD102 on different lysates with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/1,000 dilution.

Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse heart tissue lysate (no heat)
Lane 3: Mouse kidney tissue lysate (no heat)
Lane 4: Mouse kidney tissue lysate
Lane 5: Mouse spleen tissue lysate (no heat)
Lane 6: Rat spleen tissue lysate (no heat)

Lysates/proteins at 40 ug/Lane.

Predicted band size: 31 kDa
Observed band size: 45-50 kDa

Exposure time: 1 minute 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32456) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456]

Immunohistochemistry: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32456) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.