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ICAP-1 Antibody (3B2)

Novus Biologicals, part of Bio-Techne | Catalog # H00009270-M09

Novus Biologicals, part of Bio-Techne
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H00009270-M09

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ITGB1BP1 (AAH12264, 1 a.a. ~ 200 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFRKGKKRHSSSSSQSSEISTKSKSVDSSLGGLSRSSTVASLDTDSTKSSGQSNNNSDTCAEFRIKYVGAIEKLKLSEGKGLEGPLDLINYIDVAQQDGKLPFVPPEEEFIMGVSKYGIKVSTSDQYDVLHRHALYLIIRMVCYDDGLGVGKSLLALKTTDASNEEYSLWVYQCNSLEQAQAICKVLSTAFDSVLTSEKP

Specificity

Reacts with integrin beta 1 binding protein 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ICAP-1 Antibody (3B2)

Western Blot: ICAP-1 Antibody (3B2) [H00009270-M09]

Western Blot: ICAP-1 Antibody (3B2) [H00009270-M09]

Western Blot: ICAP-1 Antibody (3B2) [H00009270-M09] - Analysis of ITGB1BP1 expression in transfected 293T cell line by ITGB1BP1 monoclonal antibody (M09), clone 3B2. Lane 1: ITGB1BP1 transfected lysatE (21.8 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ICAP-1 Antibody (3B2) [H00009270-M09]

Immunocytochemistry/ Immunofluorescence: ICAP-1 Antibody (3B2) [H00009270-M09]

Immunocytochemistry/Immunofluorescence: ICAP-1 Antibody (3B2) [H00009270-M09] - Analysis of monoclonal antibody to ITGB1BP1 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: ICAP-1 Antibody (3B2) [H00009270-M09] - Detection limit for recombinant GST tagged ITGB1BP1 is 0.3 ng/ml as a capture antibody.

Applications for ICAP-1 Antibody (3B2)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against transfected lysate for western blot and recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ICAP-1

The cytoplasmic domains of integrins are essential for cell adhesion. The protein encoded by this gene binds to the beta1 integrin cytoplasmic domain. The interaction between this protein and beta1 integrin is highly specific. Two isoforms of this protein are derived from alternatively spliced transcripts. The shorter form of this protein does not interact with the beta1 integrin cytoplasmic domain. The longer form is a phosphoprotein and the extent of its phosphorylation is regulated by the cell-matrix interaction, suggesting an important role of this protein during integrin-dependent cell adhesion. [provided by RefSeq]

Long Name

Integrin Cytoplasmic Domain-associated Protein 1

Alternate Names

Bodenin, ICAP1, ITGB1BP1

Entrez Gene IDs

9270 (Human)

Gene Symbol

ITGB1BP1

Additional ICAP-1 Products

Product Documents for ICAP-1 Antibody (3B2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ICAP-1 Antibody (3B2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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