ID1 Antibody (2C7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26211
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2C7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ID1 Antibody (2C7)
Immunogen
A synthesized peptide derived from Human ID1 [UniProt P41134]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ID1 Antibody (2C7)
Western Blot: ID1 Antibody (2C7) [NBP3-26211] -
Western Blot: ID1 Antibody (2C7) [NBP3-26211] - Positive Western Blot detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate. 293 whole cell lysate, A549 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate.All lanes: ID1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 17, 16 kDa
Observed band size: 26 kDa
Immunocytochemistry/Immunofluorescence: ID1 Antibody (2C7) [NBP3-26211] -
Immunocytochemistry/Immunofluorescence: ID1 Antibody (2C7) [NBP3-26211] - Staining of Hela Cells with ID1 Antibody (2C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).Applications for ID1 Antibody (2C7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: ID1
Long Name
Inhibitor of DNA Binding 1
Alternate Names
BHLHB24, bHLHb24DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, dJ857M17.1.2, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein), ID, inhibitor of differentiation 1, Inhibitor of DNA binding 1, inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Gene Symbol
ID1
Additional ID1 Products
Product Documents for ID1 Antibody (2C7)
Product Specific Notices for ID1 Antibody (2C7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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