ID1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13286

Novus Biologicals, part of Bio-Techne
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NBP3-13286
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (90%), Porcine (90%), Rat (96%), Rhesus Macaque (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ID1 Antibodies »

Product Summary for ID1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse ID1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ID1 Antibody

Western Blot: ID1 Antibody [NBP3-13286]

Western Blot: ID1 Antibody [NBP3-13286]

Western Blot: ID1 Antibody [NBP3-13286] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with ID1 antibody (NBP3-13286) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ID1 Antibody [NBP3-13286]

Immunocytochemistry/ Immunofluorescence: ID1 Antibody [NBP3-13286]

Immunocytochemistry/Immunofluorescence: ID1 Antibody [NBP3-13286] - ID1 antibody detects ID1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ID1 stained by ID1 antibody (NBP3-13286) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10um.
Western Blot: ID1 Antibody [NBP3-13286]

Western Blot: ID1 Antibody [NBP3-13286]

Western Blot: ID1 Antibody [NBP3-13286] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ID1 antibody (NBP3-13286) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
View 2 more images

Applications for ID1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ID1

Id1 is encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

Inhibitor of DNA Binding 1

Alternate Names

BHLHB24, bHLHb24DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, dJ857M17.1.2, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein), ID, inhibitor of differentiation 1, Inhibitor of DNA binding 1, inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Gene Symbol

ID1

Additional ID1 Products

Product Documents for ID1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ID1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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