ID2 Antibody (4E12G5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37417

Novus Biologicals, part of Bio-Techne
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NBP2-37417
NBP2-37417-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4E12G5

Format

BSA Free

Concentration

1 mg/ml

View all ID2 Antibodies »

Product Summary for ID2 Antibody (4E12G5) - BSA Free

Immunogen

Purified recombinant fragment of human ID2 (AA: 1-134) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ID2 Antibody (4E12G5) - BSA Free

Western Blot: ID2 Antibody (4E12G5)BSA Free [NBP2-37417]

Western Blot: ID2 Antibody (4E12G5)BSA Free [NBP2-37417]

Western Blot: ID2 Antibody (4E12G5) [NBP2-37417] - Analysis using ID2 mAb against human ID2 recombinant protein. (Expected MW is 17.3 kDa).
Immunohistochemistry-Paraffin: ID2 Antibody (4E12G5) - BSA Free [NBP2-37417]

Immunohistochemistry-Paraffin: ID2 Antibody (4E12G5) - BSA Free [NBP2-37417]

Immunohistochemistry-Paraffin: ID2 Antibody (4E12G5) [NBP2-37417] - Analysis of rectum cancer tissues using ID2 mouse mAb with DAB staining.
Flow Cytometry: ID2 Antibody (4E12G5) - BSA Free [NBP2-37417]

Flow Cytometry: ID2 Antibody (4E12G5) - BSA Free [NBP2-37417]

Flow Cytometry: ID2 Antibody (4E12G5) [NBP2-37417] - Analysis of SK-N-SH cells using ID2 mouse mAb (green) and negative control (purple).
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Applications for ID2 Antibody (4E12G5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ID2

ID2 is encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.

Long Name

Inhibitor of DNA Binding 2

Alternate Names

GIG8

Gene Symbol

ID2

Additional ID2 Products

Product Documents for ID2 Antibody (4E12G5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ID2 Antibody (4E12G5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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