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ID4 Antibody (PCRP-ID4-1A10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20625

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-ID4-1A10

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment (around aa 62-112) of human ID4 protein

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Positive Controls: HeLa or U87 cells.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for ID4 Antibody (PCRP-ID4-1A10)

ID4 Antibody (PCRP-ID4-1A10)

ID4 Antibody (PCRP-ID4-1A10)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ID4 Mouse Monoclonal Antibody (PCRP-ID4-1A10).

Applications for ID4 Antibody (PCRP-ID4-1A10)

Application
Recommended Usage

Immunoprecipitation

1-2 ug per 100-500 ug of protein or 1ml of lysate

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ID4

Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes ina number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo-and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain theHLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA andtranscriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 (PubMed7665172)).(supplied by OMIM)

Long Name

Inhibitor of DNA Binding 4

Alternate Names

Idb4

Gene Symbol

ID4

Additional ID4 Products

Product Documents for ID4 Antibody (PCRP-ID4-1A10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ID4 Antibody (PCRP-ID4-1A10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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