IDH2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22166

Novus Biologicals, part of Bio-Techne
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NBP2-22166SS
NBP2-22166
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Guinea Pig (100%), Hamster (100%), Primate (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all IDH2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human IDH2 protein (between residues 375-452) [UniProt P48735].

Localization

Mitochondria

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IDH2 Antibody - BSA Free

Western Blot: IDH2 AntibodyBSA Free [NBP2-22166]

Western Blot: IDH2 AntibodyBSA Free [NBP2-22166]

Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody - BSA Free [NBP2-22166]

Immunocytochemistry/ Immunofluorescence: IDH2 Antibody - BSA Free [NBP2-22166]

Immunocytochemistry/Immunofluorescence: IDH2 Antibody [NBP2-22166] - 293T cells transfected with IDH2-flag expression construct were fixed and stained with anti-IDH2 antibody followed by Alexa Fluor 488 anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.
Immunohistochemistry: IDH2 Antibody - BSA Free [NBP2-22166]

Immunohistochemistry: IDH2 Antibody - BSA Free [NBP2-22166]

Immunohistochemistry: IDH2 Antibody [NBP2-22166] - Staining of IDH2 in mouse intestine.
View 2 more images

Applications for IDH2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1 - 2 ug/mL
Application Notes
In Western blot a band is observed ~48 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NBP2-22166 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IDH2

Isocitrate dehydrogenase 2, also known as IDH, IDP, oxalosuccinate decarboxylase, IDPM, and mNADP-IDH, catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH2 is imperative for metabolic regulation and energy production, primarily via the citric acid cycle. In addition, IDH2 has been implicated in tight interactions with the pyruvate dehydrogenase complex. IDH2 is localized in the mitochondria and is found in stems, roots and leaves. Recent research has found that mutations in IDH2 can block histone demethylation and result in an inability for cells to differentiate in gliomas (PMID: 22343901). Further studies have also linked IDH2 to both gallbladder and bile duct cancer (PMID: 22180306).

Alternate Names

D2HGA2, EC 1.1.1.42, ICD-M, IDH, IDHM, IDP, IDPM, isocitrate dehydrogenase [NADP], mitochondrial, isocitrate dehydrogenase 2 (NADP+), mitochondrial, mNADP-IDH, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Entrez Gene IDs

3418 (Human); 269951 (Mouse); 361596 (Rat)

Gene Symbol

IDH2

UniProt

P48735 (Human), P54071 (Mouse), P56574 (Rat)

Additional IDH2 Products

Product Documents for IDH2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IDH2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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