IDH3B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78787

Novus Biologicals, part of Bio-Techne
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NBP1-78787
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all IDH3B Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HAASRSQAEDVRVE corresponding to internal region (near N-Terminus) according to NP_777280.1.

Specificity

This antibody is expected to recognize isoform a (NP_008830.2) and isoform b (NP_777280.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IDH3B Antibody

Western Blot: IDH3B Antibody [NBP1-78787]

Western Blot: IDH3B Antibody [NBP1-78787]

Western Blot: IDH3B Antibody [NBP1-78787] - Staining of Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: IDH3B Antibody [NBP1-78787]

Western Blot: IDH3B Antibody [NBP1-78787]

Western Blot: IDH3B Antibody [NBP1-78787] (0.3ug/ml) staining of Human Skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: IDH3B Antibody [NBP1-78787]

Western Blot: IDH3B Antibody [NBP1-78787]

Western Blot: IDH3B Antibody [NBP1-78787] - Staining of Mouse Heart (A) and Mouse Muscle (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for IDH3B Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:1000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 39 kDa band observed in mouse, rat and porcine heart lysates and in human and mouse skeletal muscle lysates (calculated MW of 41.9 kDa band according to NP_777280.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IDH3B

IDH3B codes for a protein that has three isoforms, with lengths of 385, 383, and 233 amino acids, and weights of 42, 42, and 26 kDa respectively, and is a protein that is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase, which localizes to the mitochondrial matrix. Current studies are being done on several diseases and disorders related to this gene, including retinitis pigmentosa, blindness, and ataxia. IDH3B has also been shown to have interactions with MAPK6, MYC, IDH3G, PHLDA3, and IDH3A in pathways such as the TCA cycle and metabolic pathways.

Alternate Names

EC 1.1.1, EC 1.1.1.41, FLJ11043, H-IDHB, isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, isocitrate dehydrogenase 3 (NAD+) beta, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, beta subunit, Isocitric dehydrogenase subunit beta, MGC903, NAD(+)-specific ICDH subunit beta, NAD+-specific ICDH, NAD+-specific isocitrate dehydrogenase b subunit, NAD+-specific isocitrate dehydrogenase beta, RP46

Entrez Gene IDs

3420 (Human); 170718 (Mouse); 94173 (Rat)

Gene Symbol

IDH3B

UniProt

NP_008830.2 (Human)

Additional IDH3B Products

Product Documents for IDH3B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IDH3B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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