IDH3B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14114

Novus Biologicals, part of Bio-Techne
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NBP2-14114
NBP2-14114-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IDH3B Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: VKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IDH3B Antibody

Western Blot: IDH3B Antibody [NBP2-14114]

Western Blot: IDH3B Antibody [NBP2-14114]

Western Blot: IDH3B Antibody [NBP2-14114] - Analysis using Anti-IDH3B antibody NBP2-14114 (A) shows similar pattern to independent antibody NBP2-58706 (B).
Immunocytochemistry/ Immunofluorescence: IDH3B Antibody [NBP2-14114]

Immunocytochemistry/ Immunofluorescence: IDH3B Antibody [NBP2-14114]

Immunocytochemistry/Immunofluorescence: IDH3B Antibody [NBP2-14114] - Staining of human cell line A549 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: IDH3B Antibody [NBP2-14114]

Immunohistochemistry-Paraffin: IDH3B Antibody [NBP2-14114]

Immunohistochemistry-Paraffin: IDH3B Antibody [NBP2-14114] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.
View 4 more images

Applications for IDH3B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500-1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IDH3B

IDH3B codes for a protein that has three isoforms, with lengths of 385, 383, and 233 amino acids, and weights of 42, 42, and 26 kDa respectively, and is a protein that is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase, which localizes to the mitochondrial matrix. Current studies are being done on several diseases and disorders related to this gene, including retinitis pigmentosa, blindness, and ataxia. IDH3B has also been shown to have interactions with MAPK6, MYC, IDH3G, PHLDA3, and IDH3A in pathways such as the TCA cycle and metabolic pathways.

Alternate Names

EC 1.1.1, EC 1.1.1.41, FLJ11043, H-IDHB, isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, isocitrate dehydrogenase 3 (NAD+) beta, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, beta subunit, Isocitric dehydrogenase subunit beta, MGC903, NAD(+)-specific ICDH subunit beta, NAD+-specific ICDH, NAD+-specific isocitrate dehydrogenase b subunit, NAD+-specific isocitrate dehydrogenase beta, RP46

Gene Symbol

IDH3B

Additional IDH3B Products

Product Documents for IDH3B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IDH3B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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