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IDO2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45209

Novus Biologicals, part of Bio-Techne
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NBP1-45209

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-RDKTLESILHPR corresponding to C-Terminus according to NP_919270.2.

Epitope

C-Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IDO2 Antibody

Western Blot: IDO2 Antibody [NBP1-45209]

Western Blot: IDO2 Antibody [NBP1-45209]

Western Blot: IDO2 Antibody [NBP1-45209] - (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Frozen: IDO2 Antibody [NBP1-45209]

Immunohistochemistry-Frozen: IDO2 Antibody [NBP1-45209]

Immunohistochemistry-Frozen: IDO2 Antibody [NBP1-45209] - Pieces of the skin sample used to manufacture the fibroblast and keratinocyte cell banks were frozen and embedded for cryosectioning. Skin sections were stained for the immunodetection of IDO-2. Isotypic controls for IDO-2 staining are indicated in the publication. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0070408), licensed under a CC-BY license.

Applications for IDO2 Antibody

Application
Recommended Usage

Immunohistochemistry-Frozen

1:10 - 1:500

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human kidney lysates (calculated MW of 47.07 kDa band according to NP_919270.2). IHC-F usage is reported in scientific literature (PMID: 23894651).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IDO2

Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 (PubMed 17499941)).(supplied by OMIM)

Long Name

Indoleamine 2,3-dioxygenase 2

Alternate Names

Ido-2, INDOL1, Indoleamine 2,3-dioxygenase-2

Entrez Gene IDs

169355 (Human)

Gene Symbol

IDO2

UniProt

Additional IDO2 Products

Product Documents for IDO2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IDO2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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