Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70994

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1D9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Iduronate 2-Sulfatase/IDS Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IDS(NP_000193) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

59.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free

Western Blot: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9)Azide and BSA Free [NBP2-70994]

Western Blot: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9)Azide and BSA Free [NBP2-70994]

Western Blot: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free [NBP2-70994] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Iduronate 2 sulfatase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti
Immunocytochemistry/ Immunofluorescence: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free [NBP2-70994]

Immunocytochemistry/ Immunofluorescence: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free [NBP2-70994]

Immunocytochemistry/Immunofluorescence: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free [NBP2-70994] - Staining of COS7 cells.
Immunohistochemistry: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free [NBP2-70994]

Immunohistochemistry: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free [NBP2-70994]

Immunohistochemistry: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free [NBP2-70994] - Staining of paraffin-embedded Human pancreas tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.
View 12 more images

Applications for Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Iduronate 2-Sulfatase/IDS

Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. A splice variant of this gene has been described.

Alternate Names

SIDS

Gene Symbol

IDS

Additional Iduronate 2-Sulfatase/IDS Products

Product Documents for Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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