IER5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97507
![Immunocytochemistry/ Immunofluorescence: IER5 Antibody [NBP2-97507]](https://resources.bio-techne.com/images/products/IER5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97507-img0001.jpg "Immunocytochemistry/ Immunofluorescence: IER5 Antibody [NBP2-97507]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for IER5 Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human IER5 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for IER5 Antibody
Immunocytochemistry/ Immunofluorescence: IER5 Antibody [NBP2-97507]
Immunocytochemistry/Immunofluorescence: IER5 Antibody [NBP2-97507] - Immunofluorescence staining of IER5 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human IER5 polyclonal antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.Applications for IER5 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IER5
Alternate Names
immediate early response 5, immediate early response gene 5 protein, MGC102760, SBBI48
Gene Symbol
IER5
Additional IER5 Products
Product Specific Notices for IER5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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