IFIT2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15180
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Chimpanzee (94%), Orangutan (94%), Rhesus Macaque (94%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 320-370 from human IFIT2 was used as the immunogen for this antibody.
Reactivity Notes
The IFIT2 amino acid sequence used as immunogen for this antibody is 94% in White-cheeked Gibbon.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IFIT2 Antibody
![Western Blot: IFIT2 Antibody [NBP2-15180]](https://resources.bio-techne.com/images/products/IFIT2-Antibody-Western-Blot-NBP2-15180-img0004.jpg "Western Blot: IFIT2 Antibody [NBP2-15180]")
Western Blot: IFIT2 Antibody [NBP2-15180]
Western Blot: IFIT2 Antibody [NBP2-15180] - MAPL is required for early steps of immune response to Sendai virus infection. Maplfl/fl and Mapl-/- MEFs were left untreated or infected with Sendai virus (150 HAU/mL) for up to 18 hours and lysates were immunoblotted. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-00151-6), licensed under a CC-BY license.![Immunocytochemistry/ Immunofluorescence: IFIT2 Antibody [NBP2-15180]](https://resources.bio-techne.com/images/products/IFIT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15180-img0003.jpg "Immunocytochemistry/ Immunofluorescence: IFIT2 Antibody [NBP2-15180]")
Immunocytochemistry/ Immunofluorescence: IFIT2 Antibody [NBP2-15180]
Immunocytochemistry/Immunofluorescence: IFIT2 Antibody [NBP2-15180] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-IFIT2 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Western Blot: IFIT2 Antibody [NBP2-15180]](https://resources.bio-techne.com/images/products/IFIT2-Antibody-Western-Blot-NBP2-15180-img0002.jpg "Western Blot: IFIT2 Antibody [NBP2-15180]")
Western Blot: IFIT2 Antibody [NBP2-15180]
Western Blot: IFIT2 Antibody [NBP2-15180] - Analysis of IFIT2 in human K562 lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse RAW lysate probed with 4 ug/ml of IFIT2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Applications for IFIT2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5-10 ug/ml
Western Blot
2-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IFIT2
Alternate Names
cig42, G10P2IFI-54K, GARG-39, IFI-54, IFI54IFIT-2, IFI-54K, Interferon, alpha-inducible protein (MW 54kD), Interferon-induced 54 kDa protein, interferon-induced protein 54, interferon-induced protein with tetratricopeptide repeats 2, ISG54, ISG-54 K, ISG-54K, P54
Gene Symbol
IFIT2
UniProt
Additional IFIT2 Products
Product Documents for IFIT2 Antibody
Product Specific Notices for IFIT2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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