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IFIT3 Antibody (HL2468) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25515

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25515

Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2468

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for IFIT3 Antibody (HL2468) - Azide and BSA Free

Immunogen

Recombinant fragment of human IFIT3.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IFIT3 Antibody (HL2468) - Azide and BSA Free

IFIT3 Antibody (HL2468) - Azide and BSA Free

Western Blot: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] -

Western Blot: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
IFIT3 Antibody (HL2468) - Azide and BSA Free

Immunohistochemistry-Paraffin: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] -

Immunohistochemistry-Paraffin: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - IFIT3 antibody [HL2468] detects IFIT3 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human glioblastoma. IFIT3 stained by IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
IFIT3 Antibody (HL2468) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] -

Immunocytochemistry/Immunofluorescence: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - IFIT3 antibody [HL2468] detects IFIT3 protein at cytoplasm by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 5 min. Green: IFIT3 stained by IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:500. Blue: Fluoroshield with DAPI .

Applications for IFIT3 Antibody (HL2468) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFIT3

IFIT3, also known as IFIT4, IFI60, and RIGG, has been identified as a key element in the IFN-alpha anti-viral pathway. Additionally, IFIT3 binding negatively regulates the apoptotic effects of ISG54. IFIT3 is also a key target of STAT1, which is important in regulating IFN responses. One study has shown that expression of RIGG in a human monocytoma line led to growth arrest at the G1/S transition.

Alternate Names

CIG-49, GARG-49, IFI60Retinoic acid-induced gene G protein, IFIT-3, IFIT-4, IFIT4IFI-60K, interferon-induced protein with tetratricopeptide repeats 3, Interferon-induced protein with tetratricopeptide repeats 4Interferon-induced 60 kDa protein, IRG2, ISG60, ISG-60, RIG-GCIG49

Gene Symbol

IFIT3

Additional IFIT3 Products

Product Documents for IFIT3 Antibody (HL2468) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IFIT3 Antibody (HL2468) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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