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IFN-alpha 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-75556

Novus Biologicals, part of Bio-Techne
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NB100-75556
NB100-75556-0.025mg

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

The immunogen for this antibody was a sequence from 25a.a. to 186a.a. from Interferon alpha 2b(a 188a.a. long protein)

Reactivity Notes

This antibody is reactive against Human.

Localization

Secreted

Specificity

Interferon alpha 2b

Clonality

Polyclonal

Host

Chicken

Scientific Data Images for IFN-alpha 2 Antibody

Western Blot: IFN-alpha 2 Antibody [NB100-75556]

Western Blot: IFN-alpha 2 Antibody [NB100-75556]

Western Blot: Recombinant human IFNa2b as antigen. IgY dilution: 1:2000; Goat anti-IgY Fc-HRP: 1: 1000. Colorimetric method for signal development. ( anti-IFNA2 )
ELISA: IFN-alpha 2 Antibody [NB100-75556]

ELISA: IFN-alpha 2 Antibody [NB100-75556]

ELISA: Interferon alpha 2b Antibody [NB100-75556] - Recombinant protein as test antigen. Affinity-Purified anti- IFNa2b IgY as primary antibody (0.1 ug/ml), and Goat anti-IgY-HRP as 2nd antibody. ( anti-IFNA2 )

Applications for IFN-alpha 2 Antibody

Application
Recommended Usage

ELISA

1:200-1:500

Western Blot

1:200-1:500
Application Notes
ELISA: Recombinant protein as test antigen. Affinity-Purified anti- IFNa2b IgY as primary antibody (0.1 ug/ml). and Goat anti-IgY-HRP as 2nd antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2)

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFN-alpha 2/IFNA2

Interferon alpha 2b is one of the known 23 different variants of Interferon alpha 2b. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. Interferon alpha 2b is a potent immune modulator. It can either inhibit or enhance immune cell activity within the tightly regulated microenvironment of inflammation, depending upon the concentration of the cytokine and the activation stage of the cell. Interferon alpha 2B is currently used as an anticancer drug due to it's antiproliferative activity.

Long Name

Interferon alpha 2

Alternate Names

Ifa2, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2

Entrez Gene IDs

3440 (Human)

Gene Symbol

IFNA2

UniProt

Additional IFN-alpha 2/IFNA2 Products

Product Documents for IFN-alpha 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IFN-alpha 2 Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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