IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-258
![Western Blot: IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody [NB300-258]](https://resources.bio-techne.com/images/products/IFNAR1-%5Bp-Ser535-p-Ser539%5D-Antibody-Western-Blot-NB300-258-img0001.jpg "Western Blot: IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody [NB300-258]")
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Primate (100%), Rat (100%), Sheep (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 conjugated to KLH. Accession # P17181
Modification
p Ser535, p Ser539
Localization
Type I membrane protein.
Specificity
Specific for IFNAR1 protein phosphorylated at Ser535,539.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody
![Western Blot: IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody [NB300-258]](https://resources.bio-techne.com/images/products/IFNAR1-[p-Ser535-p-Ser539]-Antibody-Western-Blot-NB300-258-img0001.jpg "Western Blot: IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody [NB300-258]")
Western Blot: IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody [NB300-258]
Western Blot: IFNAR1 [p Ser535, p Ser539] Antibody [NB300-258] - Detection of Flag immunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT and 3. IFNAR1 S535A and S539A mutants. Detection with NB 300-258 is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide used as antigen but not by the dephospho form of this peptide.Applications for IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody
Application
Recommended Usage
Immunohistochemistry
1:1000
Western Blot
1:1000
Application Notes
Note: the molecular weight of the IFNNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110 - 130 kDa. Use in immunoprecipitation reported in scientific literature (PMID: 15337770).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IFN-alpha/beta R1
Long Name
Interferon alpha/beta Receptor 1
Alternate Names
IFN-aR1, IFN-bR1, IFNAR1, IFNbR1
Gene Symbol
IFNAR1
UniProt
Additional IFN-alpha/beta R1 Products
Product Documents for IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody
Product Specific Notices for IFN-alpha/beta R1 [p Ser535, p Ser539] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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