IFN-alpha/beta R2 Antibody

Western Blot: IFN-alpha/beta R2 Antibody [NBP1-31665]

Western Blot: IFN-alpha/beta R2 Antibody [NBP1-31665] - ORF54 induces degradation of IFNAR1.24 hours post transfection, 293T cells were treated with 1 ug/mL of puromycin to select for transfected cells. 48 hours post treatment, cells overexpressing vector control or FLAG-tagged MHV-68 ORFs were: Upper panels: Lysed to analyze level of IFNAR1. The membrane was subsequently stripped to demonstrate equal expression of the FLAG-ORFs and Beta-actin as controls, and IGF1Beta to demonstrate specificity of ORF54 to IFNAR1. Lower panels: The same lysates were also subjected to immunoblot against IFNAR2. The membrane was stripped twice to demonstrate equal expression of FLAG-ORFs and Beta-actin as controls. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1002292) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: IFN-alpha/beta R2 Antibody [NBP1-31665]

Immunocytochemistry/Immunofluorescence: IFN-alpha/beta R2 Antibody [NBP1-31665] - Analysis of methanol-fixed Hep3B.

Western Blot: IFN-alpha/beta R2 Antibody [NBP1-31665] -

Western Blot: IFN-alpha/beta R2 Antibody [NBP1-31665] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IFN-alpha/beta R2 antibody (NBP1-31665) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.