IFN-gamma R1/CD119 Antibody (JE40-50)

Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458]

Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Western blot analysis of IFN-gamma R1/CD119 on different lysates with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/1,000 dilution.

Lane 1: NCI-H226 cell lysate (20 ug/Lane)
Lane 2: LoVo cell lysate (20 ug/Lane)
Lane 3: HepG2 cell lysate (20 ug/Lane)
Lane 4: THP-1 cell lysate (20 ug/Lane)
Lane 5: K-562 cell lysate (20 ug/Lane)
Lane 6: HEK-293 cell lysate (20 ug/Lane)
Lane 7: MCF7 cell lysate (negative) (20 ug/Lane)
Lane 8: HL-60 cell lysate (20 ug/Lane)
Lane 9: SW620 cell lysate (20 ug/Lane)
Lane 10: Mouse liver tissue lysate (40 ug/Lane)
Lane 11: Mouse thymus tissue lysate (40 ug/Lane)

Predicted band size: 54 kDa
Observed band size: 40-100 kDa

Exposure time: 1 minute 2 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458]

Immunohistochemistry: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32458) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.