Do you have specific protocol for your IFNgR2 antibody (NBP1-90223) for immunocytochemistry (ICC)? More specifically, how do you recommend fixing the cells?

Name: IFN-gamma R2 Antibody
Brand: Novus Biologicals
SKU: NBP1-90223
Rating: 4 (1 reviews)

We have not validated this antibody for the ICC application and, therefore, cannot recommend a protocol. The antibody has been validated in IHC and WB. Your standard protocol for ICC can be found at this link. If you do use this antibody for ICC, you can send your results to us whether negative or positive (if they are tightly controlled) and qualify for the Innovator's reward. Our Innovator’s Reward™ program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product.

IFN-gamma R2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90223

(1)

Citations (1)

Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IFN-gamma R2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASPSAGFPMDFNVTLRLRAELGALHSAWVTMPWFQHYRNVTVGPPENIEVTPGEGSLIIRFSSPFDIADTSTAFFCYYVHYWEKGGIQQVKGPFRSNSISLDNLKPSRVYCLQVQAQLLWNKSNIFRVGHLSNIS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IFN-gamma R2 Antibody

Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223]

Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223] - Staining in human stomach and liver tissues using anti-IFNGR2 antibody. Corresponding IFNGR2 RNA-seq data are presented for the same tissues.

Immunocytochemistry/ Immunofluorescence: IFN-gamma R2 Antibody [NBP1-90223]

Immunocytochemistry/Immunofluorescence: IFN-gamma R2 Antibody [NBP1-90223] - Staining of human cell line SiHa shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223]

Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223] - Staining of human liver shows low expression as expected.

View 1 more images

Applications for IFN-gamma R2 Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-90223 in the following applications:

Immunocytochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFN-gamma R2

IFN gamma Receptor beta encodes the non-ligand-binding beta chain of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.

Long Name

Interferon gamma Receptor 2

Alternate Names

AF-1, IFN-gR2, IFNgammaR2, IFNGR2, IFNGT1

Entrez Gene IDs

3460 (Human)

Gene Symbol

IFNGR2

UniProt

P38484

Additional IFN-gamma R2 Products

Product Documents for IFN-gamma R2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for IFN-gamma R2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

IFN-gamma R2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90223

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all IFN-gamma R2 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for IFN-gamma R2 Antibody

Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223]

Immunocytochemistry/ Immunofluorescence: IFN-gamma R2 Antibody [NBP1-90223]

Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223]

Immunohistochemistry-Paraffin: IFN-gamma R2 Antibody [NBP1-90223]

Applications for IFN-gamma R2 Antibody

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: IFN-gamma R2

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional IFN-gamma R2 Products

Product Documents for IFN-gamma R2 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for IFN-gamma R2 Antibody