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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # IGHG4/1345

Concentration

0.2 mg/ml

Product Summary for Ig gamma-4 Chain C Region Antibody (IGHG4/1345)

Immunogen

Recombinant human Ig gamma-4 Chain C Region fragment (Uniprot: P01861)

Localization

Cytoplasmic

Marker

G4m Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54461)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Ig gamma-4 Chain C Region Antibody (IGHG4/1345)

Immunohistochemistry-Paraffin: Ig gamma-4 Chain C Region Antibody (IGHG4/1345) [NBP2-53218]

Immunohistochemistry-Paraffin: Ig gamma-4 Chain C Region Antibody (IGHG4/1345) [NBP2-53218]

Immunohistochemistry-Paraffin: Ig gamma-4 Chain C Region Antibody (IGHG4/1345) [NBP2-53218] - Formalin-fixed, paraffin-embedded Human Tonsil stained with IgG4 Monoclonal Antibody (IGHG4/1345).

Applications for Ig gamma-4 Chain C Region Antibody (IGHG4/1345)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Ig gamma-4 Chain C Region

Alternate Names

IGHG4, mmunoglobulin heavy constant gamma 4, mmunoglobulin heavy constant gamma 4 (G4m marker)

Gene Symbol

IGHG4

UniProt

Additional Ig gamma-4 Chain C Region Products

Product Documents for Ig gamma-4 Chain C Region Antibody (IGHG4/1345)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ig gamma-4 Chain C Region Antibody (IGHG4/1345)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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