IgE Antibody (RM122) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62027B

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM122

Concentration

1 mg/ml

View all IgE Antibodies »

Product Summary for IgE Antibody (RM122) [Biotin]

Immunogen

Human IgE

Specificity

This antibody reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgE Antibody (RM122) [Biotin]

ELISA: IgE Antibody (RM122) [Biotin] [NBP2-62027B] - ELISA of human immunoglobulins shows NBP2-62027B reacts only to human IgE lambda from human myeloma plasma and the IgE from human plasma. No cross reactivity with Human IgG, IgM, IgD, or IgA. The plate was coated with different immunoglobulins. NBP2-62027B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: IgE Antibody (RM122) [Biotin] [NBP2-62027B] - Sandwich ELISA using NBP2-62027B as the capture antibody (25 ng/well), and Biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA: IgE Antibody (RM122) [Biotin] [NBP2-62027B] - A titer ELISA using NBP2-62027B. The plate was coated with different amounts of human IgE. A serial dilution of NBP2-62027B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgE Antibody (RM122) [Biotin]

Application
Recommended Usage

Sandwich ELISA Capture

100 ng/well

Sandwich ELISA Detection

0.01 - 0.1 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgE

IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/ml, compared to 10 mg/ml for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.

Long Name

Immunoglobulin E

Alternate Names

IgE, immunoglobulin heavy constant epsilon

Gene Symbol

IGHE

Additional IgE Products

Product Documents for IgE Antibody (RM122) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IgE Antibody (RM122) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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